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A minimum óf 23 nucleotide possessing 100 homology to the target gene was observed to be required but not enough for efficient PTGS, and longer identical sequence is needed to initiate silencing 12, 13.Show citation Virus-Induced Gene Silencing, a Post Transcriptional Gene Silencing Method Turgay Unver 1, 2 and Hikmet Budak 1 1 Biological Sciences Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Orhanli, Tuzla, Turkey 2 Kocaeli University, Arslanbey MYO, Izmit, Turkey Show more Academic Editor: Chunji Liu Received 01 Dec 2008 Accepted 30 Mar 2009 Published 15 Jun 2009 Abstract Virus-induced gene silencing (VIGS) is one of the reverse genetics tools for analysis of gene function that uses viral vectors carrying a target gene fragment to produce dsRNA which trigger RNA-mediated gene silencing.
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